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KMID : 0829220140380060373
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Korean Journal of Oral and Maxillofacial Pathology
2014 Volume.38 No. 6 p.373 ~ p.380
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miRNA155 Inhibitory Effect on the Proliferation and Invasion of Salivary Gland Tumor Cell Line
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Chung Yon-Jae
Lee Chong-Heon
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Abstract
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The carcinogenesis mechanism of human salivary gland adenocarcinoma NOS is poorly understood. MicroRNA155(miRNA155) hasbeen involved in the carcinogenesis of many malignant tumors. The purpose of this study was to examine the role of miRNA155in tumor growth and invasion of adenocarcinoma NOS. Using SGT cells as a model for adenocarcinoma NOS, cell proliferation wasexamined by MTT assay after knocking down miRNA155 expression, and cell cycle analysis was performed. Invasive capacity by aTranswell culture assay, and miRNA155 expression in SGT cell line by RT-PCR were examined. In MTT assay, proliferation ofSGT-miRNA155 cells was decreased prominently after 96 hrs. Proliferation of SGT cells was markedly inhibited by knocking downmiRNA155, resulting from a blockade of cell cycle in the G1 phase, but apoptosis was increased about 4 folds. In adhesion assay,SGT-miRNA155 cells decreased about 60% compared to SGT cells. In invasion assay, inhibition of miRNA155 significantly suppressedthe invasive capacity of about 34% SGT cells. mRNA expression of SGT-miRNA155 cells prominently were decreased compared toSGT cells by RT-PCR. It suggested that miRNA155 could play an role in cell cycle progression and invasion in SGT cells, includingantitumor effect. These results have provided insights into the carcinogenic mechanisms and new intervention method of salivary glandadenocarcinoma NOS.
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KEYWORD
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miRNA155 Inhibitory Effect on the Proliferation and Invasion of miRNA-Invasion of Sgt.
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